Grain protein content amino acid compositions of wheat vary greatly depending on the genotype, fertilizers and environment. The objectives of this work were to determine the variation in the raw protein and amino acid composition of the grain under different type the fertilizers. The amino acids were assayed using ion-exchange chromatography after hydrolyzing with 6 M HCl for 24h at 110°C. Raw protein content from wheat grain was determined by Kjeldahl method, as Kjeldahl nitrogen multiplied with 6.25. Methionine and cystine were analyzed by using formic acid protection prior to acid hydrolysis. The research takes place in experimental field from U.S.A.M.V.B. Didactic Station from Timisoara and after that in the research lab of Soil Science and Plant Nutrition Department from Faculty of Agriculture. The experiments are stationary type, with wheat – maize – sun flower rotation. Each plot is sub-dived in four repetitions, linear, with size of 10 x 3 m (30 m2). The variety used for this experiment is Alex wheat (Triticum aestivum L.).The fertilizers used are: 20.20.0 complex mineral fertilizers, N 28 liquid foliar fertilizers, Nitrophoska 13.42.0 micro granulated fertilizers.

fertilization, wheat, raw protein, amino acids

Presentation: oral