Using NIRS technique it becomes more frequently used for determining physico-chemical properties of feed. This technique is elegant and very precise. The protein content of Medicago sativa is rich in essential amino acids, giving it a high biological value. This paper aims to highlight a way of direct analysis method undestructive protein and total nitrogen using near infrared spectrometry in conjunction with reflected attenuated total. The total nitrogen content was determinate with Kjeldahl method and using mathematical calculation was determinate the protein content. All this results we use for calibration of PerkinElmer FT-NIR Spectrometer Spectrum 100N. We have built mathematical models for Medicago sativa, based on these techniques and multivariated analysis allows the determination of an error prediction for the best protein and the total nitrogen of 0.00%.

NIR; protein; total nitrogen; non-destructive methods; feed; Medicago sativa

Presentation: oral