The paper deals with the in vitro behaviour of some explants (apex, node), prevailed from the esparcet species Onobrichis vicifolia Scop., for the  regeneration of species, their multiplication and application of a mutagen (table 1). The apex regenerated plants completely formed with multiplication V 2 and V 3 (media that proved to be the best). The node also generated neo plantlets completely confirmed with a high regenerative capacity on almost all variants.  On media with Zeatin the regeneration percentage reached 95 – 99%. The media with 2,4 D produced a callus from both explants, the biggest mass of embryogen callus, with visible embryos and a regenerative capacity were obtained from apex V 7 and V 9 .  The embryogen callus treated with mutagen medium in low concentration showed a good regenerative capacity especially on media with dimethylsulphonate (DMS).

in vitro morphogenesis; esparcet; Onobrichis vicifolia Scop.; in vitro regeneration; apex; node; diethyl sulphonate (DES); dimethylsulphonate (DMS)

Presentation: oral