EVALUATION OF LARGE THYME AND CREEPING BENTGRASS ALCOHOLIC EXTRACTS ON HUMAN HEPATOCYTE CARCINOMA CELL LINES PUBLISHEDLavinia Sarbu1*, Luminita Pirvulescu2*, Luminita Cojocariu 1
Abstract. Romania meadows are home to a great diversity of species. Some species are known to have some pytho-properties such as Thymus sp. varieties, but there are many other species that have been studied only as fodder plants such as of Agrostis sp. varieties. In this study, our aim is to analyze the antiproliferative characteristics of certain plant extracts. For this, were tested Thymus pulegioides L. and Agrostis stolonifera L. The plants alcoholic extracts were evaporated and then re-suspended in dimethyl sulfoxide. To assess the antitumor effect were used hepatic carcinoma cell line HepG2. The cells were cultivated in 90 micro liters of medium supplemented with 10 micro liters of extract in a final concentration of 25 and 50 mg/ml. The anti-proliferative effect was observed after 48 hours through spectrophotometric technique of MTT cellular proliferation. The following have been highlighted: the inhibition of cellular proliferation, dose dependency for Agrostis stolonifera products both in the case of extracts from leaves as well as those from the root, the inhibition rate being of 45% and 48% respectively. In the case of large thymus (Thymus pulegioides L.) extracts, cellular proliferation was observed only for the 50mg/ml dose, the rate of inhibition being of 37% for extracts from leaves, 39% for extracts from flowers and 26% for extracts from the stem. In conclusion, alcoholic extracts of the studied plants present therapeutic antitumor effects through the inhibition of in vivo multiplication of neoplastic cells. Further studies should be carried out for the biochemical characterization and the highlighting of the benefic effect in vivo. We believe that Locvei meadows mountains (Banat Mountains) constitute a reservoir of species with possible herbal effects and it is not yet sufficiently exploited.
Key words: Thymus pulegioides L., Agrostis stolonifera L., alcoholic extracts