INVESTIGATIONS ON THE QUANTITY OF DEZOXYRIBONUCLEIC ACID (DNA) FRAGMENTED FROM NUCLEUS OF HUMAN SPERM CELL PUBLISHEDMihaela OSTAN, Anca-Laura STĂNIA, Olga-Alina RADA, Raluca POPESCU, Delia HUŢANU email@example.com
Infertility is “a disease of the reproductive system defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse.”(Zegers-Hochschild F. et al., 2009). This paper presents an aspect of male infertility, the percent DNA fragmentation from sperm’s head and it’s clinical application. To obtain the percent of DNA fragmentation from sperm’s head we used sperm chromatin dispersion test commercialized under Halosperm test name. One of the factors involved in male infertility is the percent of DNA fragmentation from sperm’s head. The present study was made at Gynatal Clinic from Timisoara, on 25 male patients, who addressed to the Clinic for couple infertility between June 2015-May 2016. The female partner was tested and the couple’s diagnostic was idiopathic infertility. After the female partner was tested and excluded as a cause for the couple infertility, the sperm count and the percent of DNA fragmentation from sperm’s head were performed. These tests were made in order to achieve more information and to help the specialist to choose the adequate human assisted reproductive technique. After this study, we may conclude that the measurement of the percent of DNA fragmentation from sperm’s head has practical relevance. Taking in consideration the results of the percent of DNA fragmentation from sperm’s head test, the specialist can decide the most suitable human assisted reproductive technique in order to maximize the attempt.
male infertility, Halosperm, ADN
Biology applied in Agriculture