THE PLS-CV MODEL FOR DETERMINATION OF TOTAL CRUDE PROTEIN CONTENT IN FORAGES FROM A PERMANENT MEADOW (GRĂDINARI; CARAS-SEVERIN) USING NIR SPECTROSCOPY (1100-2200 NM) PUBLISHEDMonica Hărmănescu, Alexandru Moisuc
In this paper are presented our researches regarding the determination of total crude protein content (%) of forages samples, harvested in March 31 st , 2008, from a permanent meadow situated near Grădinari (Caraş Severin), using the obtained spectra between 1100-2200 nm of NIR Spectroscopy. The permanent meadow was organized in ten trials with different doses of organic and mineral fertilizations, and five replicates for each trial. The mathematical model (PLS-Cross Validation model) for total crude protein content (%) determination by NIRS method was created with the values obtained using wet chemical Kjeldahl method and those for reflectance from NIR spectra (1100-2200nm). For the statistical interpretation of obtained results was used UNSCRAMBLE software. The external calibration was made using samples harvested from the same permanent meadow, but in October, 2007.
NIR Spectroscopy; PLS-Cross Validation; total crude protein content; forages