The aim of this work was to develop a shell-less culture system for chicken embryos. There were examined the conditions required for embryonic hatching by comparing factors such as the addition of calcium lactate and optimal moisture monitoring, and the plastic wrap along with a glass were used as culture medium. Observations on the development of the chickens (Gallus gallus domesticus) embryo were made from day 1 until the hatching age of the chick, day 21. In our study, the embryos were transferred to the artificial environment after different numbers of days, starting on day 0 (without being preincubated), 24, 48, 55 and 72 hours after preincubation. A number of 10 eggs were used in each sample. As study material we used eggs from mixed breed, kept free chickens from private households. The data obtained showed that the pre-incubation period until the transfer of embryos to the culture vessel influenced the viability and success of the embryo. Transfer of embryos to the culture vessel without preincubation is not recommended because the embryos did not survive more than three days. The transfer of the embryos to the culture vessel at 24 hours led to the survival of the embryos until the fifth day, reaching the maximum mortality on the fourth day. The highest viability was obtained at 72 hours of preincubation, and when the embryo was transferred to the culture vessel after 80 hours, it was difficult to transfer without destroying the yolk membrane.

shell free culture, chichen embryos

biology

Presentation: poster