The presence of mycotoxins especially deoxynivalenol (DON) is a high concern of grain growers. Contamination in feed and foodstuffs can cause serious health problems and diseases. Six spelt wheat cultivars were cultivated in the Slovak republic under ecological farming conditions on a Haplic luvisol at the location with continental climate. Pre-crop of spelt was peas. The experiment was organized into six randomized blocks in three replicates. During 2007 and 2008 growing seasons, the concentration of DON was detected in T. spelta varieties: Bauländer Spelz, Franckenkorn, Holstenkorn, Oberkulmer Rotkorn, Rouquin and Rubiota. The liquid chromatography WATERS BREEZE equipped with binary pump WATERS 1525, RHEODYNE injector and UV detector WATERS 2487 was used with a stainless steel reverse phase 150x3,9 mm, 4 mm spherical particle C18 Nova-Pak column. The effect of varieties on DON concentration was significantly different, with the year x variety interaction also being significant. The lowest DON concentration was determined in Rouquin (165 mg.kg -1 ), whereas the highest production of mycotoxin was found in Holstenkorn and Oberkulmer Rotkorn (676,4 and 545,4 mg.kg -1 ). In three varieties (Bauländer Spelz, Franckenkorn, Oberkulmer Rotkorn) significant differences of DON concentrations between years were determined. The detected levels of DON did not exceed the maximum levels according to EU regulation 1881/2006.

Triticum spelta, Fusarium, DON, kernels

Presentation: oral