SEROLOGICAL DETECTION OF GRAPEVINE FANLEAF VIRUS (GFLV) IN AMPELOGRAPHIC COLLECTION OF USAMV IAŞI, ROMANIA PUBLISHEDEugen ULEA, Nicoleta IRIMIA, Florin Daniel LIPŞA
Grapevine fanleaf virus (GFLV) is one of the most severe virus diseases in vineyards worldwide. GFLV is a positive ssRNA virus from family Comoviridae, genus Nepovirus. Virus is transmitted by nematodes and is known as a causative agent of fanleaf degeneration of grapevine. It causes extensive leaf yellowing, stem and leaf deformation, reduced fruit quality, substantial crop loss and shortened longevity of vineyards. In 2011 a sanitary survey was conducted in ampelographic collection belonging to Agricultural Sciences and Veterinary Medicine University from Iaşi (Romania) on 50 genotypes belonging to Vitis spp. Our objective was to determine the presence and distribution of GFLV across the ampelographic collection. Leaf samples were taken during spring season from vines exhibiting virus-like symptoms or general vine decline. Three mature leaves including the petiole from different sections of the vine, keeping between the first and fifth node from the base of the vine were collected in dry, cool weather. Totally, 153 samples of symptomatic leaves from surveyed varieties were collected, put into separate plastic bags, frozen with liquid nitrogen, transported to the laboratory, and stored at −80ºC until analysed. The results of DAS-ELISA test confirm that virus was present in 11 grapevine varieties (22.0% of total) from the ampelographic collection. Infected vine cultivars with the highest OD (optical density) values were Blauerzweigelt, Newburger, Merlot, Gordan, Cioinic and Galbenă de Odobeşti.
Vitis spp.; DAS ELISA; GFLV