The spread of multidrug-resistant bacteria has become a significant cause for concern. A real and topical issue is the control and treatment of bacterial diseases, primarily caused by these bacterial mutants resistant to most available antibiotics, which is a real and pressing concern. Numerous studies concentrate on alternative or complementary antimicrobial strategies due to these facts. Antimicrobial compounds derived from natural resources, such as plant extracts, are garnering increasing interest for their activity against various microorganisms in the hope that, unlike antibiotics, they will be effective without inducing resistance. The purpose of this work is to test the antimicrobial efficacy of Thuja occidentalis (TO) extracts against Gram-negative bacteria and fungi represented by the following reference strains: Shigella flexneri (ATCC 12022), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Candida parapsilopsis (ATCC 22019) and Candida albicans (ATCC 10231), respectively. The evaluation was conducted in accordance with ISO 20776-1:2019 by measuring the loss of microbial mass using spectrophotometry to ascertain the optical density (OD). The best effect was for TO2, and it recommends it as a potential future candidate in natural products with antimicrobial activity. Our findings could allow TO2 usage in several areas, such as products for antimicrobial dermal treatments, the area being strongly affected by the increase in antimicrobial resistance to commercial products. The statistical analysis indicates that TO is highly effective against Gram-negative bacteria and Candida spp., making it a promising candidate for future research.

Thuja occidentalis, extract, Gram-negative, Candida, antimicrobial efficacy.

agronomy

Presentation: poster