IMPROVING SAMPLE PREPARATION AND DNA EXTRACTION METHOD IN APHIDS PUBLISHEDAntonia Odagiu, Ioan Oroian, Valentin Bâlteanu, Cristian Iederan, Ioan Braşovean, Claudia Balint
Improving the sample preparation, testing and improving DNA extraction methods in aphids were the aims of our paper. In this field, few researches were developed at national level, and relatively few titles are found in literature, too. Traditional sampling methods, in collection bottles were used. Sample preparation and DNA extraction were performed according to literature methodology, but technical improvements were also tested. The molecular approach of this field is relatively new and it has been developed in last decade. The improved methods obtained in aphid sample preparation and DNA extraction will facilitate the development of PCR based methods of genotyping the plant predators, globally, and development of screening methods for several interesting species, simultaneously. The importance of implementing these techniques at large scale is revealed by the accuracy of results, and also by the possibility of suitable selection of the products used for fighting against aphids.
aphids; DNA; sampling; extraction; improvement