SIMULTANEOUS DETERMINATION OF THREE ACTIVE INGREDIENTS OF A PESTICIDE, USING GAS CHROMATOGRAPHY-FLAME IONIZATION DETECTOR PUBLISHEDValdete Vorpsi, Fatos Harizaj, Vjollca Vladi
A gas chromatographic method developed and validated for simultaneous determination of spiroxamine, tebuconazole and triadimenol of a pesticide formulation ( Falcone) for quality evaluation, using a glass capillary HP5 column (30 m x 0.32 mm; 0.25 µm), temperature programming with flame ionization detector and dicyclohexylphthalate as an internal standard. The calibration graphs were found linear in the concentration range of 1 µg/mL to 1000 µg/mL for three active ingredients with correlation coefficient of 0.999, 0.999 and 0.998 respectively and co-efficient of variation for intra-day and inter-day repeatability studies at different concentration levels was found to be less than 2%. The accuracy of method ranges between 98.5% to 100.8%. Specificity were also within the acceptable range. The method is highly sensitive with LOD and LOQ as 0.5 and 2 µg/mL for spiroxamine, tebuconazole and triadimenol respectively. The developed method was successfully applied to the simultaneous quantitative analysis of spiroxamine, tebuconazole and triadimenol and therefore can be used for the routine quality control, ensuring efficiency and efficacy of the formulation for the desired purpose.
Gas chromatography, spiroxamine, simultaneous determination tebuconazole, triadimenol