In the recent years in many countries around the world, the "in vitro"  cultivation method has a wide use in studying and producing many agricultural crops, ornamental and forest plants. The solution of various problems that arise during the “in vitro” cultivation of plants as is the defining of the medium feeder, citokinins and auxines impact, selection of meristems and the optimum growth conditions, are the foundations of biotechnology today. The purpose of this study is determining of the optimal concentration of citokinins for shoot proliferation. For  experimenting  were taken from plum plants,  cv "shengjine" and as a starting material for performing experiments are used  meristems tissue  during vegetative period  and early branches of the plum during winter. Development of shoots in the specified medium along with other modifications requires that in the field to be added the citokinin – BAP (benzylaminopurine).  M-S medium with the ½ of macro & micronutrients was proved with different doses of BAP 0.5, 1, 1.5, 2 mg / l. In the same growth conditions. For the best dose of this phytohormone was judged based on the number of shoots and their length.

BAP, citokinins, "invitro" cultivation, phytohormones, shoot proliferation.

Presentation: oral