IN VITRO RESPONS OF SEVERAL CULTIVARS OF VITIS VINIFERA L ON MEDIA WITH BALANCED PHYTOHORMONE RATIO PUBLISHEDVasile Laslo, Maria Zăpârţan, Simona Vicaş
A great inconvenient of the conventional multiplication methods is that they cannot ensure a fast enough multiplication according to market needs for varieties free from viruses obtained in clonale selection programs. In vitro micro-propagation makes possible the production of healthy and uniform plants in a short time and in sufficient quantities for commercial production. The present survey has been elaborated on the purpose of establishing a protocol for in vitro multiplication for two valuable vine varieties, through the comparison of combinations of explants and culture mediums. It was carried on the in vitro conservation of the following varieties of Vitis vinifera L: Cabernet Sauvignon and Italian Riesling. The biologic material (apexes and leaf nodes from shoots forced under glass) has been in vitro multiplied on MS culture mediums suplemented with cu 3g/l vegetal coal(V1) ,BA – 1mg/l + AIA – 0.5 mg/l (V2) , BA – 1mg/l + AIA – 0.5 mg/l + 825 mg/l NH 4 NO 3 (V3), Z – 1 mg/l + AIA 0.5 mg/l (V4), Z – 1 mg/l + AIA 0.5 mg/l + 825 mg/l NH 4 NO 3 (V5). The observed parameters were: regeneration capacity based on the explant type and the medium composition; in vitro complete organogenesis; regeneration time and acclimatization aspects. The highest regeneration percent and the complete organization of neoplants has taken place on the mediums with added NH4NO3 (V3 and V5). The best multiplication took place on the zeatine mediums (Z) with or without added NH 4 NO 3 . Remarkable is also V1 with vegetal coal, which has stimulated the regeneration (but not the multiplication) of the apex of Vitis vinifera (not on nodes though). Among the two studied varieties, Cabernet Sauvignon has showed a higher regeneration percent (cca 68-75%), while Italian Riesling riches only 60 % regeneration. The in vitro regeneration time was cca. 4 months (over 120 days), based on explant and medium composition. The acclimatization capacity of the Vitis vinifera plantules depends mainly on the health of the root system and on the state of the neoplant’s organogenesis.
shoot tip; node; Cabernet Sauvignon; Riesling Italian; regeneration; organogenesis; multiplication; acclimatization