Carmen Mihaela CRISTEA, Maria OPREA, M. BERCA
The fungus Fusarium graminearum is one micromycetes than can produce significant croplosses, and wheat,specially. Fungus attack contributes to impairment of quality indictors of wheat seedand is present in micoflora responsible for the appearance of black-point attack at seeds ofwheat.Understanding the biology of pathogens gives us the information regarding the right moment toapply control measures in order to discontinue the infection development. Our studies have been orientedtoward defining the biological development parameters of fungal growth, under controlled conditions.Research has been performed in the laboratory of Mycology from Research - Development Institute forPlant Protection Bucharest and Phytopathology laboratory of the Faculty of Agriculture of the Universityof Agricultural Sciences and Veterinary Medicine of Bucharest. The biological material used was thephytopathogenic fungus Fusarium graminearum, isolated from the seeds of variety Capo wheat, camefrom” Probstdorfer Saatzucht” Romania SRL, location Modelu, Calarasi County. Infected seeds werekept wet chambers for sporulation and fungal isolation was done by direct isolation method. Fungalstrain was stored on sterilized potato dextrose agar (PDA) slants, at 4 0 C. The influences of some abioticfactors (temperature, relative atmospheric humidity, pH value and the influence of medium culture) werestudied and the results are presented in this paper. The results showed that the optimal humidity limitwas established at values over 75%. The Fusarium graminearum fungal colony development is influencedby temperature values: the lowest temperature limit for colony development was 6 0 C; the optimumtemperature needed for colony development was between 21-30 0 C; the highest temperature level may beconsidered at 36 0 C. The colonies grow fast on Malt-Agar and growth medium on Czapek'sDox-Yeastextract-Agar (CYA) growth medium (initial pH 6).
seed, fungus, biological parameters
Presentation: oral