HISTOPATHOLOGIC CHANGES OF THE SMALL INTESTINE MUCOSAE IN PIGLETS WITH ANOXIA AND PARTURITION HYPOXIA PUBLISHED

Olga Alina RADA, Liliana VASILE, H. SĂRĂNDAN, H. CERNESCU, Diana BREZOVAN, Mihaela OSTAN
The experiment took place a commercial closed circuit pig farm. From 10 still born piglets,squashed by sows and sacrificed in the first 24 hours of life, duodenum, jejunum and ileonum sampleswere harvested for the histopathological exam. The tissue samples were prepared with the classichistological techniques and were stained with haematoxylin-eosin (H.E.). 10 villi and crypts weremeasured with the analysis and image capturing programme of the Olympus C x 41 microscope. Thevascular changes were described, as well as the changes of the villosity epithelium and of the enterocyteand goblet cell aspect, and the villi mucous integrity. Glycaemia, measured before the sacrifice with aAccucek device, appreciated the parturition hypoxia level. Piglets anoxic at birth (still born) presented atthe small intestine mucosae level vascular lesions, expressed by blood and lymphatic stasis, edema inlamina propria and subepithelial in the duodenum, jejunum and ileonum. The enterocytes from thejejunum and ileonum present inversed polarity, and the other mucus secreting cells are hyperplasic. Inthe three segments of the small intestine, the villi atrophy took place, their height representing 49.5 % inthe jejunum and 35.57 % in the ileonum in comparison to the live and healthy newly-borns.During the lifeinterval at birth – 24 hours in piglets suffering from parturition hypoxia, the mucosae lesions were also ofvascular type, manifested by local ischemia, followed by reperfusion. In relation to the ischemiaseriousness in the small intestine mucosae, tissue edema, villi atrophy up to amputation, villi epitheliumdenudation and ulcers in the criptae area. Also, enterocyte hypertrophy with special nuclei migration,hypertrophy and hyperplasia of mucus secreting cells; in the lamina propria, subepithelial and in the villiaxe there was a massive leukocyte infiltration. The villi height was reduced in the three segments of thesmall intestine, the percentage of the villi height being under 400 μm.
piglets, anoxia, parturition hypoxia, villi, criptae
Presentation: oral

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