PRELIMINARY STUDY OF C. (MORPHOCARABUS) ZAWADZKII SERIATISSIMUS REITTER 1896 FROM NORTHERN ROMANIA PUBLISHEDJ. BARLOY, F. PRUNAR, Frederique BARLOY-HUBLER, S. DREANO
The study carried on C. (Morphocarabus) zawadzkii Reitter 1896, taxon belonging to the controversial supraspecific level: subsp. of C. (Morphocarabus) scheidleri Panz.; subsp. of C. (Morphocarabus) zawadzkii Kr. In a forthcoming paper carrying on 8 species from Romania and 1 from France, the marker 28Sr ensures the next regrouping: I. the super-group rothi with hampei, rothi, comptus, alutensis, kollari where C. (Morphocarabus) kollari Palliardi should be attached at rothi and not at scheidleri; II. group seriatissimus-incomptus, not positioned in relation to zawadskii and scheidleri because of samples lack; III. monilis et excellens in separates groups. However the endophallus of seriatissimus has a close form to that of C. (Morphocarabus) zawadzkii dissimilis Csiki but different from that of C. (Morphocarabus) scheidleri Panz. to which, without doubt, seriatissimus is not attached. The geographical distribution in Romania (RO) and in Ukraine (UA) was clarified (bibliographic data of variable ancientness and recent collections). The known localities (especially RO) appearing on a map. The species occurs in the Maramureș Mountains (Romania, Ukraine), Podpula (UA), Țibleș Mountains (RO) and western foothills of Rodna Mountains (RO). It cohabits with C. (Morphocarabus) incompsus Kr. 1880 in the Chornogora Mountains (UA) and perhaps in the Tibleș Mountains (RO). An analysis concerns two populations groups: the north zone (Lunca la Tisa, Bocicoel, Repedea, Ruscova, Borșa) in the Maramures County and the east zone (Romuli, Telciu) at western edge of the Rodna Mountains in the Bistrița Nasaud County. The morphometric characteristics distinguish these two groups: the individuals of the East group have higher dimensions to those of the North group. The morphometric analysis was completed with the molecular biology analysis. The molecular markers COI I, cytochrome c oxidase subunit I and 16 S rRNA clearly distinguish the northern and eastern groups with a modest variability (differences 6 base pairs for the COI I, 5 base pairs for cytochrome b), either respectively an interpopulation variability of 1.15 and 0 75%; compatible values with the literature data. The 12 S marker does not provide any distinction. In the analysis of COI I some origins of C. (Morphocarabus) incompsus Kr. (species belonging to the same group as C. (Morphocarabus) zawadzkii seriatissimus Reitter) ranks near the eastern group. The nuclear markers Wg and Pepck show the sequence positions with individuals supernumerary holding bases whose origin is discussed (insufficient samples, introgression). A possible introgression requires the study of more individuals and to identify a possible donor.
Carabus (Morphocarabus) zawadzkii seriatissimus; taxonomy, phylogeny, molecular biologyimus; taxonomy, molecular biology