THE APPEARANCE OF SCLEROTINIA SCLEROTIORUM ON GREEN BEANS AND THE EXAMINATION OF ANTIFUNGAL EFFECT OF EXTRASOL® PUBLISHEDRADUJKOV Danijela, VLAJIĆ S., MAŠIREVIĆ S., VUJIČIĆ Jovana, TARLANOVIĆ Jelena
The causer of white mold (Sclerotinia sclerotiorum (Lib.) de Bary) represents a very significant pathogen on numerous grown plant species, especially vegetables. The possibility of suppression of pathogen is very limited and the crop rotation is extremely narrow due to distinct poly-pathogenicity. Recent studies of control of white mold are aimed towards the use of biological products. During 2014 the appearance of white mycelular cover with black sclerotia was noted on ground part of the stem and first pods in the crop rotation of green beans on Zmajevo locality (45° 27′ 08" NGW, 19° 41′ 05" EGL). Sclerotia was collected and the isolation on potato dextrose agar (PDA) was performed, and after acquiring pure cultures, six isolates have been formed. Fungus mycelium was distinctively white, and during six days of cultivation at 25°C it covered the Petri dish of 90 mm in diameter, and the appearance of sclerotia on colonies’ edges was noticed on the eleventh day. With the aim of examining the biological control of S. sclerotiorum, Extrasol® (Biogenesis) was used, based on Bacillus subtilis C 13. The efficiency evaluation of the product was conducted in vitro, by transmitting of round shaped fragments, 5 mm in size, of S. sclerotiorum colonies, 7 days old, to the surface of the PDA base in which the product in 10, 15 and 20% concentration has been added, prior to the gelation. After the 7 days incubation period, the measurement of mycelia growth diameter has been performed at 25°C in cases with different concentrations and control cases. The efficiency of the product with 20% concentration was 100%, with 15% concentration it was 35.8%, and no efficiency of the product has been noted with the concentration of 10%.
Sclerotinia sclerotiorum, green beans, Bacillus subtilis