C.F. RUS1*, Georgeta POP1, Ersilia ALEXA1, Renata M. ȘUMĂLAN1, Dana M. COPOLOVICI1
Abstract. The aim of this research study is to establish the minimum inhibitory concentration (MIC) of the Salvia officinalis L. EO cultivated in the western part of Romania wich causes a fungistatic effect against Verticillium dahliae and Penicillium aurantiogriseum fungi. Also we will determine the chemical composition of this tested EO.Verticillium dahliae and Penicillium aurantiogriseum are very common in crop deposits and they are responsible for the degradation of the grains after the harvest.This research is carried for green solutions of crop protection, safe for human consumption and friendly for the environment.The antifungal activity of Salvia officinalis EO has been tested worldwide but the chemical composition and the MIC can vary due to the climate conditions, region of cultivation and the time of harvest. Further research has to be made to extract and create the optimal combination of chemical compunds from the EO that inhibits the growth of seed borne fungi. Salvia officinalis L. was grown in a temperate climate zone in Timisoara, Romania. The harvest took place in May 2014 during the blooming period after a sunny period of time. At the time of harvest Salvia officinalis L. was in the 4th year of vegetation. The tests made in vitro on CYGA medium, with additional oil at 0.25 mg•L-1; 0.5 mg•L-1; 1 mg•L-1; 5 mg•L-1; 10 mg•L-1; 15 mg•L-1 concentrations and inoculated with harvested plugs from a young mycelium, pointed out a different reaction of the fungus depending on the oil concetration. The value of the minimum inhibitory concentration (MIC) has been established after 14 days. Restauration of hyphae and mycelium of fungi was verified. The MIC value for Verticillium dahliae was 10 mg•L-1 for Salvia officinalis L. EO. Penicillium aurantiogriseum had a very similar sensitivity to the Salvia officinalis L. EO, therefore the MIC value was 10 mg•L-1 guaranteed for a period of 14 days.
Key words. Salvia officinalis L., mycelial growth inhibition, fungistatic, minimum inhibitory concentration.
Presentation: oral