Verticillium species are common in many soils and have a great number of host plants, with preference for some specific hosts (tomatoes, pepper, strawberry, sea buckthorn, potato etc.). Sea buckthorn (Hippophae rhamnoides L.) is one of the preferred host plants. Due to its capacity to produce systemic infections able to kill the host plant, verticillium wilt is considered a severe disease. The purpose of this study was the evaluation of the antifungal activity of two essential oils (thyme and juniper) and two hydrolates from spontaneous plants Filipendula vulgaris and Achillea millefolium on the growth of Verticillium sp. fungus isolated from infected roots of sea buckthorn. The essential oils of juniper and thyme and the hydrolates from Filipendula vulgaris and Achillea millefolium were tested in different doses, respectively 0.5µl, 1µl and 2µl in three replicates, there being obtained 12 variants plus the non-treated tester. The tested essential oils and hydrolates were reported as having antifungal and antibacterial effect. From this point of view are less studied the hydrolates from Filipendula vulgaris and Achillea millefolium, but they are known and used in the traditional medicine under different forms. The testing was developed in laboratory on culture media treated and inoculated with the phytopathogenic fungus Verticillium sp. isolated from infected roots of sea buckthorn. The growth of the fungus Verticillium sp. on the treated media was inhibited in different rates. The lowest inhibition rates were determined on the culture media treated with Achillea millefolium hydrolate, respectivellz 14% (at 0.5µl dose), 50% (at 1 µl) and 65% (in 2 µl); difference being very low among variants. In the case of juniper oil the inhibition rate increases, the fungus being inhibited in 57% at 0.5µl and 76% at 2µl. Thyme essential oil is highlighted in this research by a very good fungistatic activity, the inhibition being 100% in the case of 1µl and 2 µl and 94% in 0.5µl. Both essential oils and hydrolates used in this research have antifungal activity. The antifungal capacity differs from a case to other, less in the hydrolates case and greater in the case of essential oils. A considerable fungistatic activity had the thyme oil, this being proved by the maximum inhibition rate of the mycelium growth.

Verticillium sp., Achillea millefolium, Filipendula vulgaris, Juniperus communis, Thymus vulgaris, essential oils, hydrolates, sea buckthorn.

biology

Presentation: poster