For bioremediation to be successful, the bioremediation methods depend on having the rightmicrobes in the right place with the right environmental factors for degradation to occur. The rightmicrobes are bacteria or fungi, which have the physiological and metabolic capabilities to degrade thepollutants. Recent studies have reported several bacteria and filamentous fungi species with the capacityto mineralize or to degrade petroleum hydrocarbons. Bioremediation can be done on site, is often lessexpensive and site disruption is minimal, it eliminates waste permanently, eliminates long-term liability,and has greater public acceptance, with regulatory encouragement, and it can be coupled with otherphysical or chemical treatment methods. Chemical pollution of the soil environment has become a majorsource of concern. Crude oil bioremediation of soils is limited by the bacteria activity in degrading thespills hydrocarbons. In this paper are presented the results obtained in a bioremediation laboratoryexperiment. The aim of this study is to enhance the bioremediation of soils polluted with crude oil byadding the natural biodegradable product and bacterial inoculum. A natural biodegradable product andbacterial inoculum was used for total petroleum hydrocarbon (TPH) removal from an artificial pollutedsoil. Soil polluted with 50000 mg/kg of TPH was treated with 0.25%, respective 0.5% and/or bacterialinoculum to increase the biodegradability rate. Also, the soil contaminated with 100000 mg/kg of TPHwas treated with 0.5%, respective 1% and/or bacterial inoculum. The main objective of this work is toaccelerate the biodegradation processes. The enhancement of petroleum hydrocarbons degradation wasachieved under natural product treatment and bacterial inoculum. The bacterial inoculum was used toenrich indigenous microbes to enhance biodegradation rate in the green house experiment. In soilexcessively polluted with crude oil, bacterial population size in conditioned variant with Ecosol maximumdose (1%) presented values comparable to those of inoculated variants, demonstrating the protective andstimulation effect of soil bacteria, including those involved in the degradation of petroleum hydrocarbonsexercised by organic compound applied Ecosol. At each phase of the study, the natural biodegradableproduct was found to significantly enhance the biodegradation of petroleum hydrocarbons.

total number of fungi, crude oil, pollution, cambic chernozem.

Presentation: oral