Microorganism assemblage comprised of extracellular polymeric substance (EPS) and other biological elements represents the bacterial biofilm. Even though biofilm formations might help with some soil and wastewater treatment procedures, they might also put patients at risk for long-term infections and poor personal hygiene in both clinical and industrial settings. It has been noted that adding certain essential oils or extracts directly to various goods has an antibacterial and/or antioxidant impact. A growth in microbial biofilm resistance in recent years has presented significant issues for the food business in terms of contamination and shelf-life loss, as well as for the medical community in terms of treating diseases. This paper evaluates the antibacterial efficiency against the biofilm generated by Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), as well as the Minimum Biofilm Inhibitory Concentration (MBIC) of the essential oil of Origanum vulgare. The evaluation was performed by measuring the microbial biofilm using the microdilution assay, crystal violet staining, and reading of OD by spectrophotometry per ISO 20776-1:2019. Confocal microscopy was used to capture the images of S. aureus biofilm before and after treatment with the tested essential oil. The statistically evaluated results suggest a very good efficacy against S. aureus bacteria and a more limited activity against E. coli.

Origanum, anti-biofilm, Staphylococcus aureus, Escherichia coli, MBIC.

biology

Presentation: poster